278 Awardee Talk: Progesterone Regulates the Abundance of Mrnas and Proteins Involved in Polyamine Metabolism in the Sheep Endometrium

Abstract Agmatine and polyamines (putrescine, spermidine, spermine), metabolites of L-arginine, are important for development mammalian conceptuses. The objective this study was to determine if exogenous progesterone administered during the pre-implantation period, which accelerates conceptus development, alters expression mRNAs proteins involved in metabolism agmatine sheep uterus pregnancy. Mature Suffolk ewes were bred fertile rams assigned randomly be treated with daily i.m. injections either 1ml corn oil (CO;n=28) or 25mg (P4;n=20) from Day 1.5 through 8 after breeding (Day 0). Ewes hysterectomized on 9, 12, 125 gestation endometrial samples collected. candidate quantified using qPCR localized immunohistochemistry. On 9 pregnancy, AMD1 (P < 0.01), ASL SLC12A8 0.05) greater endometria P4-treated compared CO-treated ewes. MAT2B mRNA 0.001) both Days 12. gestation, tended have SAT1 0.10) In contrast, AMD1(P 0.05), OAT SMOX lower 125. Exogenous P4 appeared increase relative abundance uterine luminal glandular epithelia These results suggest that early administration may alter biosynthesis period pregnancy potential ‘programming’ effects manifested late